SARS-2 Origin: WIV1 pk RaTG13
Zhiyan-Le, 2021-03-30.
https://zhiyanleback.blogspot.com/p/sars-2-origin-wiv1-pk-ratg13-zhiyan-le.html
The WHO China mission report on SARS-2 origin stated: SARS-CoV-2 also shares a
96.2% homology with a sequence of a strain of coronavirus (RaTG13) previously
identified by genetic sequencing from a horseshoe bat sample (Rhinolophus
species) and to a lesser extent with a strain isolated from pangolins. The
RaTG13 virus sequence is the closest known sequence to SARS-CoV-2. #
That is done by single gene alignment. However, when the virus and ACE2 are
running, they work by Codon-protein, that is, three base genes as one unit.
Clearly, some Codon studies may have to be in place, which may lead to different
solutions.
For example, we have two gene strings:
TTTCCAGGTAACAAACCAACG
CTTTCCAGGTAACAAACCAAC
Using single gene alignment method, they have 91% identity:
Identities: 20/22(91%) | |
Gaps: 1/22(4%) | |
Query 1 TTTCCAGGTAACAAACCAACG | 21 |
|||||||||||||||||||| | |
Sbjct 1 CTTTCCAGGTAACAAACCAAC | 21 |
And applying a study on the Codon level which is a reality in running, they have
a 0% similarity:
TTT | CCA | GGT | AAC | AAA | CCA | ACG |
CTT | TCC | AGG | TAA | CAA | ACC | AAC |
WIV1 pk RaTG13
Now there are two possible origins re SARS-2: RaTG13, mentioned above, which
cannot directly jump to humans; another is WIV1 (GenBank: KF367457), a
lab-constructed nCov, which was warned by NAS (US National Academy of Science)
in March 2016 that it can directly jump to humans and cause huge pandemic and
economic losses.
Take NC_045512 as basic sample (collected from Wuhan patient, short name WH-01),
and do some alignment studies on the Codon level, the similarities are like
this:
SARS-2: Codon Frequence, Distribution and Distance (original data: NIH GenBank, by 2021-02-22) | |||||||||||||||
WH-01 | WIV1 | RaTG13 | WIV1 from WH-01 | RaTG13 from WH-01 | |||||||||||
1st | 2nd | 3rd | 1st | 2nd | 3rd | 1st | 2nd | 3rd | 1st | 2nd | 3rd | 1st | 2nd | 3rd | |
A | 3075 | 2903 | 3137 | 3061 | 2855 | 2738 | 2828 | 3087 | 3011 | -14 | -48 | -399 | -247 | 184 | -126 |
C | 2132 | 1610 | 1862 | 2051 | 2135 | 1876 | 1599 | 1838 | 2073 | -81 | 525 | 14 | -533 | 228 | 211 |
G | 1525 | 1909 | 2517 | 2389 | 1629 | 2276 | 1921 | 2466 | 1459 | 864 | -280 | -241 | 396 | 557 | -1058 |
T | 3425 | 3735 | 2641 | 2602 | 3484 | 3213 | 3603 | 2560 | 3408 | -823 | -251 | 572 | 178 | -1175 | 767 |
avrg | 2539.25 | 2525.75 | 2487.75 | -13.5 | -51.5 | ||||||||||
align w WH-01: | 0.99468 | 0.97972 |
The ACGT are base genes; 1st-2nd-3rd refers to first, second and third base
gene. The distance is calculated in a very simple but strait forward way: WIV1
(or RaTG13) value minus WH-01.
Result 1: WIV1 has a 0.99468 similarity to basic sample (WH-01, Wuhan
patient sample), much higher than that of RaTG13 (0.97972).
Result 2: From WH-01, WIV1 has a distance (-13.5), much shorter than that
of RaTG13 (-51.5).
Other factors:
Sept.18 2019, in Wuhan metro area, the PRC authorities hosted a real-time
exercise re pandemic emergencies, for which Wuhan Institute of Virology provided
SARS-2 samples and relevant testing/treatment tech-skills. And the RaTG13
provider, Wuhan Institute of Virology, stated that they do not have RaTG13
live-samples but genomic sequences; Meanwhile, WIV1 is their lab-constructed
SARS-2, meaning that the institute can provide it at any time anywhere.
Plus, as said above, WIV1 can but RaTG13 cannot directly jump to humans.
Based on the above, lab-made WIV1 is the most possible origin re SARS-2, not
RaTG13 or other bats. (note: The PRC authorities have closed the area where
RaTG13 was collected. WHO report did not clearly say if they demanded to go
there and repeat the collection, a common method to test if a sample can be held
true.)
PS:
The WHO report used some fake data made by PRC institutes. See:
https://zhiyanleback.blogspot.com/2021/03/who-sars-2-origin-report-uses-fake-data.html
https://zhiyanleback.blogspot.com/p/who-report-uses-fake-data-grand-failure.html
(in Chinese language).
=-==========
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